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ONtrack®

NGS-based chimerism monitoring on Oxford Nanopore Technologies

Benefits

  • Compatible with ONT sequencing
  • Chimerism levels between 0.5–1%
  • Results for a single sample in <3 hours

Description

Chimerism monitoring after hematopoietic stem cell transplantation enables prediction of an upcoming relapse. Enhanced sensitivity of chimerism assays allows for earlier detection, thereby increasing the time available for treatment. ONtrack® is an in-development solution designed to enable rapid and sensitive chimerism monitoring using Oxford Nanopore Technologies (ONT) platforms. Based on the well-established NGStrack assay, ONtrack uses the same 34 biallelic indel markers and integrates seamlessly into the ONT sequencing workflow. The combination of ONT speed and TRKengine® data analysis enables same-day results for urgent or high-throughput chimerism research.

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Specifications

Key features

  • Rapid results: Results for a single sample in under 3 hours

  • Flexible throughput: Process 1 to 96 samples depending on your research needs

  • Enhanced sensitivity: Detect chimerism levels as low as 0.5–1%, with strong linearity (R² = 0.999)

  • Same marker set as NGStrack (34 indel markers covering 18 chromosomes, including sex chromosomes)

  • Compatible with:

    • ONT sequencing platforms (e.g., MinION, GridION)

    • TRKengine® analysis software

    • NGS-TurboPrep for library preparation

  • Small lab footprint: Ideal for labs looking for fast, cost-effective sequencing options

Workflow

The ONtrack workflow includes two rounds of PCR using NGStrack primers and ONT-compatible indices, followed by streamlined library preparation with NGS-TurboPrep. Sequencing runs on ONT platforms (e.g., MinION) and data is analyzed in TRKengine® with minimal hands-on time.

Step Duration
Target generation ~30 minutes
Barcoding & preparation ~45 minutes
Sequencing ~30 minutes/sample
Data analysis <5 minutes
Total time ≤ 3 hours/sample

Currently in development.
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