ONtrack

Specifications

Chimerism monitoring after hematopoietic stem cell transplantation enables prediction of an upcoming relapse. Enhanced sensitivity of chimerism assays allows for earlier detection, thereby increasing the time available for treatment. ONtrack is a nanopore-based solution for chimerism monitoring, developed as an extension of the trusted NGStrack assay. It uses 34 bi-allelic indel markers and combines rapid, scalable ONT sequencing with streamlined analysis in TRKengine®. ONtrack offers a fast, flexible alternative to Illumina-based workflows, ideal for labs seeking same-day results with lower equipment investment.

Key features

• Rapid results: Results for a single sample in under 3 hours

• Flexible throughput: Process 1 to 96 samples depending on your research needs

• Enhanced sensitivity: Detect chimerism levels with sensitivity <0.5%

• Same marker set as NGStrack (34 indel markers covering 18 chromosomes, including sex chromosomes)

Workflow

The ONtrack workflow includes two rounds of PCR using NGStrack primers and ONT-compatible indices, followed by streamlined library preparation with NGS-TurboPrep. Sequencing runs on ONT platforms (e.g., MinION) and data is analyzed in TRKengine with a turnaround time of less than 3 hours per sample.

Currently in development.
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