GenDx > GenDx Products > NGS HLA Typing > Amplification > NGSgo®-AmpX v2/AmpX v3

NGSgo®-AmpX v2/AmpX v3

Singleplex HLA amplification 11 loci

Benefits

  • Optimized version of AmpX
  • Single loci
  • RUO & CE-IVD (IVDR)

Description

NGSgo-AmpX v2 and NGSgo-AmX v3 are optimized versions of NGSgo-AmpX with adjusted cycling protocol, colored primer pellet, and a convenient enzyme mastermix included in the kit. AmpX v2 uses the GenDx-LongMix enzyme, and AmpX v3 uses GenDx-LongZyme.

Available for the amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 (AmpX v2 & v3) and -G (AmpX v2). Primers can be purchased in combined-loci kits or individually. AmpX v2 and v3 are compatible with NGSgo library preparation reagents.

AmpX v2 is compatible with Illumina and Ion Torrent platforms. AmpX v3 is compatible with Illumina platforms.

NGSgo-AmpX v2 is available as RUO, CE-marked IVD (IVDR), and Health Canada IVD. NGSgo-AmpX v3 is available as RUO and CE-marked IVD (IVDR).

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61 Items

Product Status Reactions Catalog

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DRB3/4/5, DQB1, DQA1, DPB1, DPA1

Components
Nuclease free water
NGSgo®-AmpX HLA-A
NGSgo®-AmpX HLA-B
NGSgo®-AmpX HLA-C
NGSgo®-AmpX HLA-DRB1
NGSgo®-AmpX HLA-DRB3
NGSgo®-AmpX HLA-DRB4
NGSgo®-AmpX HLA-DRB5
NGSgo®-AmpX HLA-DQA1
NGSgo®-AmpX HLA-DQB1
NGSgo®-AmpX HLA-DPA1
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
CE-IVD 96 7934662 Favorite

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1

Components
Nuclease free water
NGSgo®-AmpX HLA-A
NGSgo®-AmpX HLA-B
NGSgo®-AmpX HLA-C
NGSgo®-AmpX HLA-DRB1
NGSgo®-AmpX HLA-DQB1
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
CE-IVD 96 7934462 Favorite

NGSgo-AmpX v3 HLA-A

Components
Nuclease free water
NGSgo®-AmpX HLA-A
GenDx-LongZyme
CE-IVD 96 7933162 Favorite

NGSgo-AmpX v3 HLA-B

Components
Nuclease free water
NGSgo®-AmpX HLA-B
GenDx-LongZyme
CE-IVD 96 7933262 Favorite

NGSgo-AmpX v3 HLA-C

Components
Nuclease free water
NGSgo®-AmpX HLA-C
GenDx-LongZyme
CE-IVD 96 7933362 Favorite

NGSgo-AmpX v3 HLA-DRB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DRB1
GenDx-LongZyme
CE-IVD 96 7933662 Favorite

NGSgo-AmpX v3 HLA-DQB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DQB1
GenDx-LongZyme
CE-IVD 96 7933562 Favorite

NGSgo-AmpX v3 HLA-DPB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
CE-IVD 96 7933462 Favorite

NGSgo-AmpX v3 HLA-DRB3/4/5

Components
Nuclease free water
NGSgo®-AmpX HLA-DRB3
NGSgo®-AmpX HLA-DRB4
NGSgo®-AmpX HLA-DRB5
GenDx-LongZyme
CE-IVD 96 7933762 Favorite

NGSgo-AmpX v3 HLA-DPA1

Components
Nuclease free water
NGSgo®-AmpX HLA-DPA1
GenDx-LongZyme
CE-IVD 96 7933962 Favorite

NGSgo-AmpX v3 HLA-DQA1

Components
Nuclease free water
NGSgo®-AmpX HLA-DQA1
GenDx-LongZyme
CE-IVD 96 7933862 Favorite

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1, DPA1, DQA1, DRB3/4/5

Components
Nuclease free water
NGSgo®-AmpX HLA-A
NGSgo®-AmpX HLA-B
NGSgo®-AmpX HLA-C
NGSgo®-AmpX HLA-DRB1
NGSgo®-AmpX HLA-DRB3
NGSgo®-AmpX HLA-DRB4
NGSgo®-AmpX HLA-DRB5
NGSgo®-AmpX HLA-DQA1
NGSgo®-AmpX HLA-DQB1
NGSgo®-AmpX HLA-DPA1
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
RUO 96 7334662 Favorite

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1

Components
Nuclease free water
NGSgo®-AmpX HLA-A
NGSgo®-AmpX HLA-B
NGSgo®-AmpX HLA-C
NGSgo®-AmpX HLA-DRB1
NGSgo®-AmpX HLA-DQB1
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
RUO 96 7334462 Favorite

NGSgo-AmpX v3 HLA-A

Components
Nuclease free water
NGSgo®-AmpX HLA-A
GenDx-LongZyme
RUO 96 7333162 Favorite

NGSgo-AmpX v3 HLA-B

Components
Nuclease free water
NGSgo®-AmpX HLA-B
GenDx-LongZyme
RUO 96 7333262 Favorite

NGSgo-AmpX v3 HLA-C

Components
Nuclease free water
NGSgo®-AmpX HLA-C
GenDx-LongZyme
RUO 96 7333362 Favorite

NGSgo-AmpX v3 HLA-DRB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DRB1
GenDx-LongZyme
RUO 96 7333662 Favorite

NGSgo-AmpX v3 HLA-DQB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DQB1
GenDx-LongZyme
RUO 96 7333562 Favorite

NGSgo-AmpX v3 HLA-DPB1

Components
Nuclease free water
NGSgo®-AmpX HLA-DPB1
GenDx-LongZyme
RUO 96 7333462 Favorite

NGSgo-AmpX v3 DRB3/4/5

Components
Nuclease free water
NGSgo®-AmpX HLA-DRB3
NGSgo®-AmpX HLA-DRB4
NGSgo®-AmpX HLA-DRB5
GenDx-LongZyme
RUO 96 7333762 Favorite

NGSgo-AmpX v3 HLA-DPA1

Components
Nuclease free water
NGSgo®-AmpX HLA-DPA1
GenDx-LongZyme
RUO 96 7333962 Favorite

NGSgo-AmpX v3 HLA-DQA1

Components
Nuclease free water
NGSgo®-AmpX HLA-DQA1
GenDx-LongZyme
RUO 96 7333862 Favorite

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7771662 Favorite

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7971662 Favorite

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371662 Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7771462 Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7971462 Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371462 Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770162 Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970162 Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370162 Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770262 Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970262 Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370262 Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770362 Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970362 Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370362 Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770662 Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970662 Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370662 Favorite

NGSgo®-AmpX v2 HLA-DRB1 Whole Gene

Components

HLA-DRB1 Whole Gene (except part of intron 1)
Nuclease-Free H2O
GenDx-LongMix

 RUO 96 7370622 Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770762 Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970762 Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370762 Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770862 Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970862 Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370862 Favorite

NGSgo®-AmpX v2 HLA-DQB1 Whole Gene

Components

HLA-DQB1 whole gene Amp
Nuclease Free H2O
GenDx-LongMix
Enhancer

 RUO 96 7370512 Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770562 Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970562 Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370562 Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970962 Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770962 Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370962 Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770462 Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970462 Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370462 Favorite

NGSgo®-AmpX v2 DPB1 Whole Gene One (prototype)

Components

HLA-DPB1 F1 Amp

 N/A 96 7270412 Favorite

NGSgo®-AmpX v2 HLA-G

Components

HLA-G Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371062 Favorite

NGSgo®-AmpX v2 HLA-E (prototype)

Components

HLA-E Amp
Nuclease Free H2O
GenDx-LongMix

 N/A 96 7271262 Favorite

NGSgo®-AmpX v2 HLA-F (prototype)

Components

HLA-F F1 Amp
Nuclease Free H2O
GenDx-LongMix

 N/A 96 7271962 Favorite

Specifications

NGSgo-AmpX v2 and v3 feature exactly the same HLA gene coverage as NGSgo-AmpX. The whole gene is amplified for the Class I HLA genes HLA-A, B and C. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified. For -DRB1 and -DQB1 there are also options with more extended coverage amplifying the whole (or almost the whole) gene.

Fig 1. NGSgo-AmpX v2/AmpX v3 HLAGeneSuite (kit containing 11 loci) amplification coverage

 

Fig 2. NGSgo-AmpX v2 whole gene amplification coverage

 

To be supplied by the user:

  • Cold block
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge
  • Vortex
  • PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system

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