NGSgo®-AmpX v2

Specifications

NGSgo-AmpX v2 features exactly the same HLA gene coverage as NGSgo-AmpX. The whole gene is amplified for the Class I HLA genes HLA-A, B and C. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified. For -DRB1 and -DQB1 there are also options with more extended coverage amplifying the whole (or almost the whole) gene.

Fig 1. NGSgo-AmpX v2 HLAGeneSuite (kit containing 11 loci) amplification coverage

Fig 2. NGSgo-AmpX v2 whole gene amplification coverage

To be supplied by the user:

• Cold block
• Pipettes and pipette tips with hydrophobic filters
• Thermal cycler
• Microcentrifuge
• Vortex
• PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
• Agarose gel electrophoresis system