GenDx > GenDx Products > NGS HLA Typing > Amplification > NGSgo®-AmpX v2

NGSgo®-AmpX v2

Singleplex amplification primer sets including polymerase enzyme

Benefits

  • Optimized version of AmpX
  • Single loci
  • RUO & CE-IVD

Description

NGSgo-AmpX v2 is an optimized version of NGSgo-AmpX with adjusted cycling protocol, colored primer pellet, and a convenient enzyme mastermix (GenDx-LongMix) included in the kit. Available for the amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 and -G. Primers can be purchased in combined-loci kits or individually. They are compatible with NGSgo library preparation reagents and all common sequencing platforms. NGSgo-AmpX v2 primer sets are available as RUO, CE-marked IVD, and Health Canada IVD.

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40 Items

Product Status Reactions Catalog

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7771662 Favorite

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7971662 (IVDR)
7871662 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
HLA-DRB3 Amp
HLA-DRB4 Amp
HLA-DRB5 Amp
HLA-DPA1 Amp
HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371662 Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7771462 Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7971462 (IVDR)
7871462 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

HLA-A Amp
HLA-B Amp
HLA-C Amp
HLA-DRB1 Amp
HLA-DQB1 Amp
HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371462 Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770162 Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970162 (IVDR)
7870162 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-A

Components

HLA-A Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370162 Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770262 Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970262 (IVDR)
7870262 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-B

Components

HLA-B Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370262 Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770362 Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970362 (IVDR)
7870362 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-C

Components

HLA-C Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370362 Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770662 Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970662 (IVDR)
7870662 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DRB1

Components

HLA-DRB1 Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370662 Favorite

NGSgo®-AmpX v2 HLA-DRB1 Whole Gene

Components

HLA-DRB1 Whole Gene (except part of intron 1)
Nuclease-Free H2O
GenDx-LongMix

 RUO 96 7370622 Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770762 Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970762 (IVDR)
7870762 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DRB3/4/5

Components

HLA-DRB3 Amp
HLA-DRB4 Amp
HLA DRB5 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370762 Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770862 Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970862 (IVDR)
7870862 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DQA1

Components

HLA-DQA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370862 Favorite

NGSgo®-AmpX v2 HLA-DQB1 Whole Gene

Components

HLA-DQB1 whole gene Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370512 Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 IVD Canada 96 7770562 Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 CE-IVD 96 7970562 (IVDR)
7870562 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DQB1

Components

HLA-DQB1 Amp
Nuclease Free H2O
GenDx-LongMix

 RUO 96 7370562 Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970962 (IVDR)
7870962 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770962 Favorite

NGSgo®-AmpX v2 HLA-DPA1

Components

HLA-DPA1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370962 Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 IVD Canada 96 7770462 Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7970462 (IVDR)
7870462 (IVDD) 		Favorite

NGSgo®-AmpX v2 HLA-DPB1

Components

HLA-DPB1 Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7370462 Favorite

NGSgo®-AmpX v2 DPB1 Whole Gene One (prototype)

Components

HLA-DPB1 F1 Amp

 N/A 96 7270412 Favorite

NGSgo®-AmpX v2 HLA-G

Components

HLA-G Amp
Nuclease free H2O
GenDx-LongMix

 CE-IVD 96 7871062 Favorite

NGSgo®-AmpX v2 HLA-G

Components

HLA-G Amp
Nuclease free H2O
GenDx-LongMix

 RUO 96 7371062 Favorite

NGSgo®-AmpX v2 HLA-E (prototype)

Components

HLA-E Amp
Nuclease Free H2O
GenDx-LongMix

 N/A 96 7271262 Favorite

NGSgo®-AmpX v2 HLA-F (prototype)

Components

HLA-F F1 Amp
Nuclease Free H2O
GenDx-LongMix

 N/A 96 7271962 Favorite

Specifications

NGSgo-AmpX v2 features exactly the same HLA gene coverage as NGSgo-AmpX. The whole gene is amplified for the Class I HLA genes HLA-A, B and C. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified. For -DRB1 and -DQB1 there are also options with more extended coverage amplifying the whole (or almost the whole) gene.

Fig 1. NGSgo-AmpX v2 HLAGeneSuite (kit containing 11 loci) amplification coverage

 

Fig 2. NGSgo-AmpX v2 whole gene amplification coverage

 

To be supplied by the user:

  • Cold block
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge
  • Vortex
  • PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system

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