NGSgo®-AmpX KIR

Allele-level KIR typing by NGS

Benefits

  • 8 different KIR genes
  • Includes enzyme mastermix
  • Compatible with LibrX and IndX

Description

NGSgo®-AmpX KIR goes beyond presence/absence typing as it allows for genotyping at the allele level by NGS. NGSgo-AmpX KIR provides reagents for whole-gene amplification of 8 different KIR genes, and includes GenDx-LongMix enzyme. It is compatible with NGSgo® library preparation for Illumina and analysis in NGSengine. Available as RUO.

See specifications

Catalog

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Product Status Reactions Catalog

NGSgo®-AmpX KIR 2DL1, 2DL2, 2DL3, 2DL4, 3DL1, 3DS1, 3DL2, 3DL3

Components

Nuclease free water
NGSgo®-AmpX KIR 2DL1
NGSgo®-AmpX KIR 2DL2
NGSgo®-AmpX KIR 2DL3
NGSgo®-AmpX KIR 2DL4
NGSgo®-AmpX KIR 3DL1
NGSgo®-AmpX KIR 3DL2
NGSgo®-AmpX KIR 3DL3
NGSgo®-AmpX KIR 3DS1
GenDx-Longmix

ruo 24 7372102 Favorite

Specifications

NGSgo-AmpX KIR enables whole-gene amplification of eight KIR genes from 5’ UTR to 3’ UTR, allowing for genotyping at high-to-allelic resolution.

NGSgo-AmpX KIR kit includes primers for amplification of the following genes, each amplified separately: KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR3DL1, KIR3DS1, KIR3DL2, and KIR3DL3.

Amplification coverage

To be supplied by the user:

  • Cold block or ice
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge
  • Vortex
  • PCR tubes or PCR plate (use thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system
  • Qubit® DNA BR Assay Kit (Q32853; Life Technologies
  • Qubit® Fluorometer (Q33216; Life Technologies)
  • Qubit® Assay Tubes (Q32856; Life Technologies)
  • Library preparation and sequencing reagents (see Instructions for Use of ‘NGSgo® workflow compatible with Illumina’ (document no. 2342000 and 2342001)

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