NGS-Turbo®

Specifications

High-resolution HLA typing can be performed on multiple platforms, however, options are limited when time is of the essence. The MinION sequencers from ONT provide long-read sequencing within a short timeframe, allowing quick sequencing of 1 sample. The sequencing time depends on the Oxford Nanopore Technologies device you use and can take as little as 20 minutes.

With NGS-Turbo® high-resolution typing can be achieved within 3 hours.

amplification of HLA loci

NGS-TurboAmp offers fast amplification of all 11 HLA loci (HLA-A, B, C, DQA1, DQB1, DPA1, DPB1, DRB1 and DRB3/4/5) using a whole-gene approach and results in high resolution.

library preparation

With NGS-TurboPrep, library preparation can be performed in just 30 minutes. No fragmentation is required and intermission is possible at any time point.

sequencing & data analysis

Finally, the sample can be sequenced on Oxford Nanopore sequencers and the data can be directly analyzed and typed with NGSengine® Turbo.

NGS-Turbo is an expansion of GenDx’ current high-resolution HLA typing portfolio.