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NGS-Turbo®

High-resolution Nanopore HLA typing within 4 hours

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Benefits

  • Nanopore sequencing
  • 11 loci whole gene
  • 1-tube library preparation

Description

With a turnaround time of less than four hours and an easy and robust workflow, NGS-Turbo® is designed for HLA typing in situations where time is a critical factor.

The NGS-TurboAmp kit offers 11 HLA loci using a whole-gene approach. The extremely quick 1-tube library preparation with NGS-TurboPrep requires no fragmentation and can be safely paused at any time. After nanopore sequencing, the data can be directly analyzed with NGSengine® Turbo.

NGS-Turbo is expected to launch as RUO in Q2 2023.

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Catalog

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Specifications

High-resolution HLA typing can be performed on multiple platforms, however, options are limited when time is of the essence. The MinION sequencers from ONT provide long-read sequencing within a short timeframe, allowing quick sequencing of 1 sample.

With NGS-Turbo® high-resolution typing can be achieved within 4 hours.

 

amplification of HLA loci

 

NGS-TurboAmp offers fast amplification of all 11 HLA loci (HLA-A, B, C, DQA1, DQB1, DPA1, DPB1, DRB1 and DRB3/4/5) using a whole-gene approach and results in high resolution.

 

library preparation

With NGS-TurboPrep, library preparation can be performed in just 30 minutes. No fragmentation is required and intermission is possible at any time point.

 

sequencing & data analysis

Finally, the sample can be sequenced on Oxford Nanopore sequencers and the data can be directly analyzed and typed with NGSengine® Turbo.

NGS-Turbo is an expansion of GenDx’ current high-resolution HLA typing portfolio.

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