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NGS-ProntoFLX

NGS-ProntoFLX genotyping assays

Whole-gene coverage, HLA typing, ONT

Benefits

  • Whole-gene amplification
  • Compatible with ONT devices
  • Available as prototype

Description

NGS-ProntoFLX are singleplex and duplex genotyping assays compatible with Oxford Nanopore Technologies (ONT) sequencing devices. With optimized primer design and full-gene coverage, these assays combine the flexibility of singleplex or duplex formats, together with the speed and accuracy required for reliable high-res HLA typing. Featuring a ready-to-use amplification mixture, sequencing takes only 10 minutes per sample, and up to 96 samples can be processed in parallel.

Currently available as prototypes: HLA-A, HLA-B (singleplex), and HLA DQA1/DQB1 (duplex).

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Specifications

NGS-ProntoFLX

NGS-ProntoFLX are singleplex and duplex assays designed for speedy high-resolution sequencing of HLA loci using ONT platforms.

Turnaround time DNA to results

NGS-ProntoFLX

Key features

  • Currently available as prototypes: NGS-ProntoFLX HLA-A, NGS-ProntoFLX HLA-B, NGS-ProntoFLX DQA1/DQB1 

  • Whole-gene coverage

  • 96-sample throughput per run

  • Ready-to-use amplification mixture

  • 10 minutes sequencing per sample, same-day results when using ONT platforms

  • Compatible with ONT sequencing platform

  • Data analysis with NGSengine

Whether you prefer long-read or short-read sequencing, this assay integrates seamlessly into your workflow, delivering high-quality data with rapid turnaround.

Currently in development.
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