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NGS-ProntoPrep

Library preparation for ONT

Benefits

  • Barcoded library prep for ONT (RUO)
  • Fast workflow in 1.5–2 hours
  • 1 to 96 samples

Description

NGS-ProntoPrep offers a complete, easy-to-use barcoded library preparation solution specifically designed for high-resolution HLA typing on Oxford Nanopore Technologies (ONT) devices. Designed for speed and accuracy, the workflow takes just 1.5 to 2 hours and is easily scalable for 1 to 96 samples.

For a complete ONT workflow, simply amplify your DNA with NGSgo-ProntoAmp or NGSgo-ProntoFLX, prepare your library with NGS-ProntoPrep, sequence, and analyze your data with NGSengine.

NGS-ProntoPrep is available as RUO.

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NGS-Pronto®Prep

Components

Nuclease-free water

AMPure XP beads

End Prep Mix

Ligase Mix

Binding Solution

Elution Buffer

Wash Buffer

10x Ligation Buffer

Ligase Enzyme

Barcode Plate 1

 

 

 RUO 96 9303623 Favorite

Specifications

Library preparation

The library preparation process is highly efficient and performed entirely at room temperature, allowing for a flexible workflow that can be easily paused. Depending on the number of samples, the procedure takes just 1.5 to 2 hours to complete.

Because the protocol skips fragmentation, it yields long, intact amplicons that enable full phasing. The kit is highly scalable, accommodating 1 to 96 samples per run, with 96 unique barcodes available. Sample pooling is performed conveniently in the presence of beads, and the kit supports a maximum of 12 libraries (1 to 96 samples per library).

Compatibility

Compatible with R10.4.1 flow cells, NGS-Pronto can be sequenced on MinION Mk1B, Mk1D, and GridION sequencers from ONT, delivering rapid results in as little as 10-15 minutes per sample. The assay is to be used with compatible consumables from ONT, including EXP-NBA114 and EXP-AUX003.

NGS-Pronto has been validated to be compatible with MinKNOW versions 24.06, 24.11, 25.05, and 26.01.
MinKNOW version 26.01 is only comtabible with NGSengine version 4.2 or higher.

Amplification and analysis

The NGS-ProntoPrep kit is designed to seamlessly follow NGSgo-ProntoAmp or NGSgo-ProntoFLX amplification. The resulting data is optimized for fast, accurate interpretation using NGSengine®.

Turnaround time for 12 samples < 8 hours:

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