KIR typing using ONT
KIR CompleteMX is our in development assay for comprehensive typing of Killer-cell Immunoglobulin-like Receptor (KIR) genes using Oxford Nanopore Technologies (ONT) long-read sequencing. Designed to provide full gene coverage across all relevant KIR loci, the assay aims to streamline library prep and enable in-depth KIR genotyping with NGSengine® analysis.
KIR genes, are a family of highly polymorphic genes expressed on natural killer (NK) cells and subsets of T cells. These receptors interact with HLA class I molecules and play a central role in regulating immune responses. Variability in KIR gene content and alleles is associated with outcomes in hematopoietic stem cell transplantation, infectious disease susceptibility, autoimmunity, and reproductive success.
The KIR CompleteMX assay amplifies all 15 known KIR genes and one of the two pseudogenes, in three multiplex reactions, followed by pooling and library preparation for ONT long-read sequencing. The long-read strategy enables better resolution of complex gene structures and haplotypes. Data is analyzed in NGSengine, supporting confident genotyping through an intuitive interface.
Full KIR gene panel: 2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 2DP1, 3DL1, 3DL2, 3DL3, 3DS1, 3DP1
Multiplexed amplification
All targets amplified in 3 multiplex tubes, reducing hands-on time and sample handling
Long-read optimized
Developed for Oxford Nanopore Technologies, enabling complete gene resolution and phasing
Analysis software: NGSengine
Development phase: Prototype
KIR CompleteMX is currently in development.
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