Posters
Training and Materials
ASHI 2026
GenDx Scientific Posters shared at ASHI meeting 2026- NGS-Capto-HLA: a new long-fragment based hybrid capture technology, Mattijs Punt, Anna Hill, Sjoerd Creutzburg, Johan Crins, Pascal van der Weele, .
- NGSgo-ProntoFLX: Flexible HLA Amplification of all 11 classical HLA loci, Mattijs Punt, Michelle de Ridder, Bo Rood, Pascal van der Weele, .
- Expanding NGS-Turbo with ABO subtyping, E. ter Steege, L. van Valburg, M.de Graaf, I. Oldfield, M. Menechella, P. van der Weele, .
- Early-access testing of SOtrack: HLA-informed donorderived cell-free DNA monitoring, Jolinde van Strien, Cynthia Kramer, Bram Luiken, Bo Rood, Lara van der Klugt, Lisa Rond, Pascal van der Weele, .
EFI 2026
GenDx Scientific Posters shared at EFI 2026- Tracing of residual DNA when reusing MinION flow cells with a barcoded version of NGS-Turbo, E. ter Steege, B. Rood, S. Creutzburg, P. van der Weele, .
- NGSgo-ProntoFLX: Single- or Duplex HLA Amplifications for Confirmatory Typing or Disease Association, Mattijs Punt, Michelle de Ridder, Bo Rood, Pascal van der Weele, .
- ONtrack: chimerism monitoring employing third-generation sequencing, Jolinde van Strien, Maaike Rijkers, Lisa Rond, Pascal van der Weele, .
- Development of a digital PCR-based chimerism monitoring assay, Jolinde van Strien, Bram Luiken, Styn Franken, Casper van Herwaarden, Pascal van der Weele, .
ASHI 2025
- Automation of barcoded NGS-Turbo® HLA-typing using INTEGRA MIRO CANVAS, Minique de Castro, Imogen Oldfield, Sjoerd Creutzburg, Bram Luiken, Pascal van der Weele, Maarten Penning, .
- A singleplex HLA typing approach for nanopore sequencing, Mattijs Punt, Eline ter Steege, Michelle de Ridder, Pascal van der Weele, .
- NGS-Capto-HLA: Long-Frament Hybrid Capture-Based HLA-Typing using Oxford Nanopore Technologies Sequencing, Mattijs Punt, Sjoerd Creutzburg, Anna Hill, Johan Crins, Juliette de Vries, Marcel van de Streek, Joris Albers, Maaike Rijkers, Pascal van der Weele, .
- Facilitating high-throughput HLA typing: Single-well, low-volume amplification with full exon coverage of 11 HLA loci, Johan Crins, Cindy Spruit, Pascal van der Weele, .
- KIR Complete MX: Multiplexed whole gene genotyping of all KIR genes using ONT sequencing, Bram Luiken, Maaike Rijkers, Imogen Oldfield, Anna Hill, Juliette Cloosterman-de Vries, Pascal van der Weele, .
- Robust qPCR assay for validating effective isolation of cell-free DNA, Jolinde van Strien, Bram Luiken, Bo Rood, Lara van der Klugt, Eleni Draina, Pascal van der Weele, .
EFI 2025
- Validation of an NGS-based HLA typing assay, Cindy Spruit1, Jules Petit1, Erica Kremer1, Thomas Vandewalle2, Laurie Cordonnier2, Lucie Begert2, Bérangère Vasseur2, Silvia Comis2, Caroline Chevalier2, Hanneke Merkens1, Pascal van der Weele1, Maarten Penning1, .
- A non-interventional clinical study on the performance, Maaike Rijkers1, Johan Crins1, Bram Luiken1, Nemanja Suvajac2, Andreas Heinold3, Mateusz Mrozowski4, Pascal van der Weele1, .
- NGS-Capto-HLA Long-Fragment Hybrid Capture Based, Mattijs Punt1, Sjoerd Creutzburg1, Anna Hill1, Johan Crins1, Juliette de Vries1, Marcel van de Streek1, Joris Albers1, Jos Drabbels2, Dave Roelen2, Maaike Rijkers1, Pascal van der Weele1, .
- Automated NGS-Turbo® workflow using the INTEGRA MIRO, Minique de Castro, Bart Valkenburg, Sjoerd Creutzburg, Eline ter Steege, Bram Luiken, Pascal van der Weele, .
ASHI 2024
- High-throughput, high-resolution HLA typing using NGS-Pronto nanopore sequencing - a multicentre performance study, Eline ter Steege, Sjoerd Creutzburg, Bart Valkenburg, Anna Hill, Marcel van de Streek, Joris Albers, Loes van de Pasch, Pascal van der Weele, .
- A novel HLA loss assay revealing partial HLA loss, Maaike Rijkers, Job Geerligs, Thijs Finnegan, Lisa Rond, Pedro Mikel Requejo Olaizola, David Monzón Casado, María Dolores de Juan Echavarri, Loes van de Pasch, Maarten Penning, Sake van Wageningen, .
- Sensitive and fast NGS-based chimerism monitoring using ONT sequencing, Maaike Rijkers, Bart Valkenburg, Job Geerligs, Pascal van der Weele, .
- An HLA genotype-informed dPCR assay for monitoring donor-derived cfDNA, Bram Luiken, Lara van der Klugt, Bo Rood, Monica Sorbini, Silvia Deaglio , Tiziana Vaisitti, Bart Valkenburg, Freek Manders, Eleni Draina, Sake van Wageningen, .
ASHI 2022
- Rapid, robust and reliable high resolution HLA typing of 11 HLA loci using ont minion sequencing, Pascal van der Weele, Sjoerd Creutzburg, Bart Valkenburg, Marcel van de Streek, Maarten Penning, .
- Direct vs indirect indexing for NGSBased Chimerism Monitoring, Maaike Rijkers, Pascal van der Weele, Eleni Draina, Johan Crins, Job Geerligs, Loes van de Pasch, Maarten Penning, Erik Rozemuller, .
- Optimizing gene coverage for HLA class II gene sequencing, Sake van Wageningen, Bart Valkenburg, Erik Rozemuller,, .
EFI 2022, Hybrid
- A multiplex whole-gene HLA typing strategy evaluation on various NGS platforms: both long reads (PacBio, MinION), and short reads (Illumina, Ion Torrent, MGI)., Sake van Wageningen, Pascal van der Weele, Bart Valkenburg, Helma de Bruin, Erik Rozemuller, Maarten Penning, .
- Allele-level, whole gene blood group typing with PacBio HiFi sequencing to identify new alleles, Maaike Rijkers1, Loes A.L. van de Pasch1, Daniel Alvarez1, Bart Valkenburg1, Masja de Haas2, Peter C. Ligthart2, Barbera Veldhuisen2, C. Ellen van der Schoot3, Erik H. Rozemuller1, Maarten Penning1, .
- High-resolution HLA haplotypes inference and estimation in a panel of 304 mother-child pairs, Daniel Alvarez1, Loes van de Pasch1, Gideon Hönger2,3,4, Stefan Schaub2,3,4, Erik Rozemuller1, .
- Rapid HLA typing using ONT MinION sequencing, Pascal van der Weele, Sjoerd Creutzburg, Bart Valkenburg, Maarten Penning, .
- Whole Gene KIR genotyping on a commercially available human HLA variation reference panel, Bram Luiken, Fereshte Dadkhodaie, Loes van de Pasch, Maarten Penning, .
ASHI 2021, Hybrid
- A multiplex whole-gene HLA typing strategy evaluation on various platforms: both long reads (PacBio, ONT), and short reads (Illumina, Ion Torrent) , Diya Gao, Sake van Wageningen, Helma de Bruin, Maarten Penning GenDx, Utrecht, The Netherlands, .
- NGStrack® performance and informativity analysis of 154 samples, Pascal van der Weele, Job Geerligs, Jannetje Kooij, Johan Crins, Robert Welleweerd, Loes van de Pasch, Maarten T. Penning, Erik H. Rozemuller, .
EFI 2021, Virtual
- KIR allele level genotyping of a Japanese healthy individual cohort, , P150.
- NGStrack: Chimerism monitoring by means of next-generation sequencing, , P205.
- Development of an independent sequencing workflow for confirmation of homozygous HLA typing results., , P209.
- High-resolution HLA typing with NGSgo-MX6-1 by HiFi NGS data of a PacBio Sequel II platform, , P287.
- The MR1 gene encompasses at least 6 alleles, , P291.
ASHI 2020, Virtual
- High resolution HLA typing with NGSgo-MX6-1 and PacBio HiFi sequencing, Van Wageningen S et al., P182.
ASHI 2019, Pittsburgh, Pennsylvania
- Chimerism monitoring by qPCR, a robust performance from 0.05 to 100%, Bouwmans EE et al., P296.
- Automation of the NGSgo Library Preparation for 96 and 192 Samples on the Biomek i7 Workstation, Luiken B et al., P312.
- A new NGS-based typing strategy for whole-gene HLA-DRA, Van de Pasch LAL et al., P314.
EFI 2019, Lisbon, Portugal
- Combining the Advantages of NGS Long and Short Reads for HLA and KIR Typing, Rozemuller EH et al., P182.
- Whole-Gene NGS-Based High-Resolution Genotyping Strategy for HLA-DPB1, Bouwmans EE et al., P190.
- Replacement of Pre-Transplant DNA with Cell Line DNA in Real-Time PCR Chimerism Monitoring Experiments, Van Wageningen S et al., P201.
ASHI 2018, Baltimore, USA
- Validation of the automated NGSgo Library Preparation - Workflow for HLA Typing on the Biomek 4000, Luiken B et al., P092.
- Chimerism Monitoring by Next-Generation Sequencing: a Multiplatform Comparison, Bouwmans EE et al., P101.
- Performance of a Multiplex PCR Amplification for NGS-Based Typing of Six HLA Genes, Vand de Pasch LAL et al., P102.
EFI, 2018, Venice, Italy
- Automation of the NGSgo Library Preparation - Workflow for HLA typing on the Biomek 4000 Workstation, Luiken B et al., P129.
- Automatic NGS HLA Data Analysis Using NGSignition and NGSserver, Rozemuller EH et al., P130.
- NGS-based KIR typing at high resolution: challenges and solutions for the KIR framework genes, Van de Pasch LAL et al., P141.
- A novel NGS-based high-resolution genotyping strategy for HLA-E, Van de Pasch LAL et al., P145.
