NGSengine® Software

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Is NGSengine CE-IVD marked?

Yes, NGSengine is marked as CE-IVD
(EU/EEA, Switzerland, Norway, Turkey, South-Africa).

What are the minimum & recommended system requirements?

System requirements:

Minimum System Requirements

  • Windows 10 64-bit
  • Microsoft .NET Framework 4.7.2. (included in latest Windows updates)
  • 4 GB RAM
  • 1 GB free disk space*

Recommended System Requirements

  • Windows 10 64-bit
  • Microsoft .NET Framework 4.7.2.
  • Multi core processor
  • 8 GB RAM
  • 1 GB free disk space*
  • Internet connection

* hard disk space is for installation and running of NGSengine only, so excluding space for data storage

Which file formats can be analysed by NGSengine?

NGSengine® loads files of the .FASTA, .FASTQ and .FNA format.

What is the minimum number of reads per locus?

There is no exact minimum because it depends on a number of factors like the heterozygosity of the gene, the insert sizes of the library, and the quality of the data.

We have observed optimal results when there are at least 15,000 reads per locus. If you sequence 5 loci per sample this means 75,000 reads per sample. If you have less reads, NGSengine may still obtain an unambiguous typing result, depending on your sample.

In general, more reads will lead to better phasing and less ambiguities.

What is the minimum read depth per locus?

The read depth is the amount of reads that cover one single position. We have observed optimal results when the read depth throughout the locus is at least 500 reads. A lower read depth can also produce optimal results, depending on the sample.

What is the latest version of NGSengine?

The latest version is 2.16.0, released on 9 January 2020.

IMGT/HLA Database, Release 3.38.0

You can check your version by clicking <Help> and <About NGSengine®>