NGSgo®-AmpX MICA, MICB

Whole-gene amplification of MICA and MICB  

  

 

New!

GenDx now offers the newly developed product NGSgo-AmpX MICA, MICB*. This kit includes amplification primers for whole-gene (Fig 1) amplification of MICA. As a bonus to stimulate MICB research, amplification primers for MICB have been added to the kit at no additional costs. The kit also includes a polymerase enzyme for amplification of both genes.

 

Figure 1.  A. NGSgo-AmpX MICA whole-gene amplification

               B. NGSgo-AmpX MICB whole-gene amplification

 

Background

The MHC Class I chain-related genes A (MICA) and B (MICB) are located on chromosome 6, close to HLA-B (Fig 2).

 

Figure 2. MHC region on chromosome 6.

 

MICA and MICB genes are similar in structure to HLA Class I, but they do not bind peptides and do not engage with the T-cell receptor. Instead they engage with an activating receptor, NKG2D, expressed on the surface of NK cells and certain T cells (Fig 3). This interaction stimulates the cytolytic activity and cytokine production of these cells, and thereby influences immunological responses.

 

Figure 3. MHC class I and MICA molecules, interaction with their respective receptor on a T cell.

 

In the field of transplantation, MICA has been studied more widely than MICB. In solid organ transplantation, anti-MICA antibodies have been found to be a risk factor for both acute and chronic rejection. In stem cell transplantation, MICA mismatching has been associated with graft-versus-host disease, relapse, and overall mortality. Different studies have reported conflicting results though, therefore the effect of MICA donor-recipient mismatching remains controversial.  In disease association studies, certain MICA polymorphisms have shown to be associated with various diseases including ankylosing spondylitis, inflammatory bowel disease, and diabetes.

 

Order now

Be on the cutting edge of transplantation research and order NGSgo-AmpX MICA, MICB today!

   Group   

Product description

No. of tests

Cat. No.

AmpX RUO*

NGSgo®-AmpX MICA, MICB

96

2341372

 

*For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

 

References

  • Cox ST et al. Tissue Antigens. 2014 Sep;84(3):293-303.
  • Chen D et al. Carcinogenesis. 2014 Dec;35(12):2633-42.
  • Gavlovsky PJ et al. 2016 Nov;77(11):1084-1091.
  • Carapito R et al. Blood. 2016 Oct 13;128(15):1979-1986.
  • Carapito R et al. Front Immunol. 2017 Mar 27;8:368.
  • Baranwal AK, et al. Front Immunol. 2017 Feb 28;8:182.