You can stop the SBTexcellerator protocol:
- After amplification PCR
Store overnight at 15°C in thermocycler or 4°C in fridge. Continue with ExoI/SAP clean-up preferably within 24 hours but definitely within a week after amplification.
- After clean-up by ExoI/SAP
Store overnight at 15°C in thermocycler or 4°C in fridge. Store for 1-2 weeks at 4°C. Store for >2 weeks at -20°C.
- After sequencing PCR
Store for 1-2 days at 15°C in thermocycler or 4°C in fridge.
- After clean-up by Sephadex
Store for 1-2 days at 4°C. Store for >2 days at -20°C. After a longer period of storage it is recommended to denature the reactions by putting them in a thermocycler for 2 min at 98°C and afterwards directly transfer the reactions to a cooling block or ice before running the plate on the sequencing instrument.
IMPORTANT: During storage, always keep your plates sealed to prevent evaporation!