Yes, it is possible to manually enter informative markers through one of the following two routes:
– Make a setup for a typing experiment including recipient and donor in KMRengine, perform the experiment and import the qPCR data into KMRengine. After approval of the data, return to the homescreen and select the recipient in the drop-down menu described above. The informative markers found in the typing will be displayed in the lower left screen. You can either select these markers for tracking by clicking on them or manually add additional markers using the button. Select the recipient in the ‘Reference Sample’ part of the screen and fill in details on your post sample. Then select KMRtrack on the top of the screen. Once you export this setup for tracking, the selected markers will be saved in your transplant file.
– If you already have typing data but have no KMRengine record of this, it is possible to manually set-up a tracking experiment without the use of imported KMRtype data. To do this, fill in the ‘Donor’ and ‘Recipient’ fields and select the recipient in the ‘Reference Sample’ field. Subsequently fill in the data for your post-transplant sample in the ‘Post-sample’ field. You can now manually add markers via the button. Once you export this setup for tracking, the selected markers will be saved in your transplant file.