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NGStrack® HLA loss

Detection of HLA loss by NGS

NGSgo-ProntoFLX is a single Locus HLA typing to maximize flexibility on ONT

Benefits

  • 15 informative STR markers in 1 tube
  • Complementary with the NGStrack® workflow
  • Fast data analysis with TRKengine®

Description

With the NGStrack® HLA loss kit, GenDx offers a dedicated NGS solution for the early detection of partial to complete loss of the MHC in post-transplant research. Accurate detection of this loss is a valuable tool for researching relapse mechanisms and studying cellular dynamics post-transplantation.

The easy-to-use, amplicon-based workflow includes 15 Short Tandem Repeat (STR) markers multiplexed in one tube. It is designed to be run alongside standard NGStrack® chimerism monitoring or can be used as a standalone assay in your research workflow. After Illumina paired-end sequencing, the data is directly analyzed and managed with TRKengine® software.

NGStrack® HLA loss kit is currently available as prototype.

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Specifications

Workflow

Using 15 highly variable STR markers located on chromosome 6p21, the assay provides high informativity compared to using SNPs.

 

The NGStrack® HLA loss assay uses an optimized workflow, minimizing hands-on time

  • Amplification: Fast setup, requiring less than 10 minutes. The 15 STR markers are amplified in a single tube, with PCR taking approximately 55 minutes. A recipient and donor pre-sample should be included with every experiment.
  • Library preparation: NGSgo® indices are added seamlessly during the amplification reaction, followed by a single bead clean-up step using GenDx AMPure XP beads (approximately 30 minutes). Monitoring libraries can be pooled with genotyping libraries and other NGSgo® or NGStrack® libraries before sequencing.
  • Sequencing: Compatible with Illumina sequencing platforms.
  • Data analysis: FastQ files are loaded into TRKengine®, which takes less than 5 minutes to generate results. The assay has a detection limit of >1% non-HLA recipient chimerism and distinguishes standard chimerism from complete or partial loss of the MHC from in research samples.

 

Software

Data analysis is performed with our user-friendly TRKengine® software, utilizing the dedicated NGStrack® HLA loss module (available as an additional software purchase).

 

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