Your best solution for high-throughput Sanger HLA typing
SBTessenz® assays will bring your high-throughput HLA typing to the next level.
With our expertise in Sequencing-Based Typing you can rely on us to get the results you really need.
Focusing on the peptide binding domain we have developed SBTessenz® assays
for identification of HLA-A, B, C, DQB1 and DRB1 alleles.
- 96-well plate format
- Primers dried in plate, dye included
- One round of sequencing
- Analysis by SBTengine®
- Various DNA sources
-including buccal swabs
SBTessenz is available as Research Use Only (RUO)
For class I (HLA-A, -B and -C) exon 2 and 3 are amplified. For class II (HLA-DQB1 and -DRB1) exon 2 is amplified.
The assay set-up provides an efficient combination of generic sequencing primers and Group-Specific Sequencing Primers (GSSPs) in a 96-well plate format which can be ran in parallel to accomplish enhanced ambiguity resolution in one round of sequencing. Data analysis with our trusted SBTengine® software gives you reliable HLA assignments.
HLA-A, -B and -C:
HLA-DQB1 and -DRB1:
SBTessenz® is available as Research Use Only (RUO)
SBTessenz® consists of two types of 96-well primer plates.
1) The 96-well AMP primer plate:
each well contains dried amplification primer mix sufficient for one HLA locus specific amplification
2) The 96-well SEQ primer or Group-Specific Sequencing primer (GSSP) plates:
each well contains dried sequencing primer mix sufficient for one sequencing reaction
Sufficient Taq PCR CORE kit (Qiagen) is supplied free of charge with every order of SBTessenz
|High Throughput||SBTessenz® HLA-A (96) RUO||96||1340101|
|SBTessenz® HLA-B (96) RUO||96||1340201|
|SBTessenz® HLA-C (96) RUO||96||1340301|
|SBTessenz® HLA-DQB1 (96) RUO||96||1340501|
|SBTessenz® HLA-DRB1 (96) RUO||96||1340601|
How to order?
For ordering SBTessenz® you may contact your local GenDx HLA-specialized distributor or contact us directly.