Simplify HLA sequencing by automated sample preparation on  TECAN Freedom EVO NGS workstation


A high-resolution dataset of HLA typing is ideal for compatibility testing. NGS technology provides high-resolution answers in high-throughput HLA typing.

The Automation of the NGSgo workflow on the TECAN Freedom EVO NGS workstation helps to minimize the risk of cross-contamination and increases the reproducibility of HLA typing library preparation for 48 samples with a possible extension for up to 96 samples.

The platform uses advanced air displacement pipetting technology, enabling precise eight-channel pipetting. It also includes three INHECO CPAC thermal devices, INHECO Thermoshake heated shaker, a 96-position magnetic plate separator and a Robotic Manipulator Arm. In addition, the compact worktable offers storage space for up to 12 tip boxes, allowing longer unattended runs.

User-friendly TouchTools™ interface guides the operator through instrument set-up and library preparation, reducing operator-to-operator variability for highly reproducible sequencing-ready libraries.

Automated steps include amplicon pooling, fragmentation and adapter ligation, bead clean-ups and size selection, indexing PCR setup and library pooling. The quantification, normalization and indexing PCR is performed in an offline thermocycler.   

The workflow can be fully automated by integrating a Tecan microplate reader and an on-deck thermocycler. These would allow for automated amplicon-quantification, normalization and indexing PCR cycling, significantly increasing walk-away time.

 

For more information, click on the resources below.

 

                                              Freedom EVO Brochure              Poster

 

 

Do you want to know more how you can start using GenDx NGSgo Workflow on Freedom EVO?

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