SBTessenz®


Your best solution for high-throughput Sanger HLA typing

SBTessenz® assays will bring your high-throughput HLA typing to the next level.
With our expertise in Sequencing-Based Typing you can rely on us to get the results you really need.
Focusing on the peptide binding domain we have developed SBTessenz® assays
for identification of HLA-A, B, C, DQB1 and DRB1 alleles.

 

Your benefits

  • 96-well plate format
  • Primers dried in plate, dye included
  • One round of sequencing
  • Analysis by SBTengine®
  • Various DNA sources
    -including buccal swabs

 

 

SBTessenz is available as Research Use Only (RUO)

 

Amplification Strategy

For class I (HLA-A, -B and -C) exon 2 and 3 are amplified. For class II (HLA-DQB1 and -DRB1) exon 2 is amplified.

The assay set-up provides an efficient combination of generic sequencing primers and Group-Specific Sequencing Primers (GSSPs) in a 96-well plate format which can be ran in parallel to accomplish enhanced ambiguity resolution in one round of sequencing. Data analysis with our trusted SBTengine® software gives you reliable HLA assignments.

 

 

HLA-A, -B and -C:

  • Generic sequencing primers (exon 2 and 3)
  • 2 GSSPs especially designed for frequent genotypes

 

 

HLA-DQB1 and -DRB1:

  • Generic sequencing primers (exon 2)
  • GSSPs especially designed for frequent genotypes

 

 

 

 

Available kits

SBTessenz® is available as Research Use Only (RUO)

SBTessenz® consists of two types of 96-well primer plates.

1) The 96-well AMP primer plate:

each well contains dried amplification primer mix sufficient for one HLA locus specific amplification


2) The 96-well SEQ primer or Group-Specific Sequencing primer (GSSP) plates:

each well contains dried sequencing primer mix sufficient for one sequencing reaction

Sufficient Taq PCR CORE kit (Qiagen) is supplied free of charge with every order of SBTessenz

 

Group Product Description No. of Tests Cat. No.
High Throughput SBTessenz® HLA-A (96) RUO 96 1340101
  SBTessenz® HLA-B (96) RUO 96 1340201
  SBTessenz® HLA-C (96) RUO 96 1340301
  SBTessenz® HLA-DQB1 (96) RUO 96 1340501
  SBTessenz® HLA-DRB1 (96) RUO 96 1340601

How to order?

For ordering SBTessenz® you may contact your local GenDx HLA-specialized distributor or contact us directly.

You can place your order by email: order@gendx.com

If you have any questions or need our assistance do not hesitate to contact us.
Call +31 (0)30 252 3799 or E-mail support@gendx.com

Get Support

Have questions about your experiment, data analysis or our products?

 

Visit our support pages

HLA Training School

Want to stay updated and refresh your knowledge on HLA, SBT and NGS workflow and data analysis?

 

 

 

 

 

 

Register for a session