GenDx > GenDx Products > NGS HLA Typing > Amplification > NGSgo®-AmpX v2/AmpX v3

NGSgo®-AmpX v2/AmpX v3

Singleplex amplification primer sets including polymerase enzyme

Benefits

Optimized version of AmpX
Single loci

Description

NGSgo-AmpX v2 and NGSgo-AmX v3 are optimized versions of NGSgo-AmpX with adjusted cycling protocol, colored primer pellet, and a convenient enzyme mastermix included in the kit. AmpX v2 uses the GenDx-LongMix enzyme, and AmpX v3 uses GenDx-LongZyme.

Available for the amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 (AmpX v2 & v3) and -G (AmpX v2). Primers can be purchased in combined-loci kits or individually. They are compatible with NGSgo library preparation reagents and all common sequencing platforms.

NGSgo-AmpX v2 primer sets are available as RUO, CE-marked IVD, and Health Canada IVD. NGSgo-AmpX v3 primer sets are available as RUO and CE-marked IVD.

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Catalog

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61 Items

Product		Status	Reactions	Catalog
NGSgo-AmpX v3 HLA-A, B, C, DRB1, DRB3/4/5, DQB1, DQA1, DPB1, DPA1 Components	CE-IVD	96	7934662	Favorite
NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1 Components	CE-IVD	96	7934462	Favorite
NGSgo-AmpX v3 HLA-A Components	CE-IVD	96	7933162	Favorite
NGSgo-AmpX v3 HLA-B Components	CE-IVD	96	7933262	Favorite
NGSgo-AmpX v3 HLA-C Components	CE-IVD	96	7933362	Favorite
NGSgo-AmpX v3 HLA-DRB1 Components	CE-IVD	96	7933662	Favorite
NGSgo-AmpX v3 HLA-DQB1 Components	CE-IVD	96	7933562	Favorite
NGSgo-AmpX v3 HLA-DPB1 Components	CE-IVD	96	7933462	Favorite
NGSgo-AmpX v3 HLA-DRB3/4/5 Components	CE-IVD	96	7933762	Favorite
NGSgo-AmpX v3 HLA-DPA1 Components	CE-IVD	96	7933962	Favorite
NGSgo-AmpX v3 HLA-DQA1 Components	CE-IVD	96	7933862	Favorite
NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1, DPA1, DQA1, DRB3/4/5 Components	RUO	96	7334662	Favorite
NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1 Components	RUO	96	7334462	Favorite
NGSgo-AmpX v3 HLA-A Components	RUO	96	7333162	Favorite
NGSgo-AmpX v3 HLA-B Components	RUO	96	7333262	Favorite
NGSgo-AmpX v3 HLA-C Components	RUO	96	7333362	Favorite
NGSgo-AmpX v3 HLA-DRB1 Components	RUO	96	7333662	Favorite
NGSgo-AmpX v3 HLA-DQB1 Components	RUO	96	7333562	Favorite
NGSgo-AmpX v3 HLA-DPB1 Components	RUO	96	7333462	Favorite
NGSgo-AmpX v3 DRB3/4/5 Components	RUO	96	7333762	Favorite
NGSgo-AmpX v3 HLA-DPA1 Components	RUO	96	7333962	Favorite
NGSgo-AmpX v3 HLA-DQA1 Components	RUO	96	7333862	Favorite
NGSgo®-AmpX v2 HLAGeneSuite (11 loci) Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp HLA-DRB3 Amp HLA-DRB4 Amp HLA-DRB5 Amp HLA-DPA1 Amp HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7771662	Favorite
NGSgo®-AmpX v2 HLAGeneSuite (11 loci) Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp HLA-DRB3 Amp HLA-DRB4 Amp HLA-DRB5 Amp HLA-DPA1 Amp HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7971662	Favorite
NGSgo®-AmpX v2 HLAGeneSuite (11 loci) Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp HLA-DRB3 Amp HLA-DRB4 Amp HLA-DRB5 Amp HLA-DPA1 Amp HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7371662	Favorite
NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1 Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7771462	Favorite
NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1 Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7971462	Favorite
NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1 Components HLA-A Amp HLA-B Amp HLA-C Amp HLA-DRB1 Amp HLA-DQB1 Amp HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7371462	Favorite
NGSgo®-AmpX v2 HLA-A Components HLA-A Amp Nuclease Free H2O GenDx-LongMix	IVD Canada	96	7770162	Favorite
NGSgo®-AmpX v2 HLA-A Components HLA-A Amp Nuclease Free H2O GenDx-LongMix	CE-IVD	96	7970162	Favorite
NGSgo®-AmpX v2 HLA-A Components HLA-A Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370162	Favorite
NGSgo®-AmpX v2 HLA-B Components HLA-B Amp Nuclease Free H2O GenDx-LongMix	IVD Canada	96	7770262	Favorite
NGSgo®-AmpX v2 HLA-B Components HLA-B Amp Nuclease Free H2O GenDx-LongMix	CE-IVD	96	7970262	Favorite
NGSgo®-AmpX v2 HLA-B Components HLA-B Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370262	Favorite
NGSgo®-AmpX v2 HLA-C Components HLA-C Amp Nuclease Free H2O GenDx-LongMix	IVD Canada	96	7770362	Favorite
NGSgo®-AmpX v2 HLA-C Components HLA-C Amp Nuclease Free H2O GenDx-LongMix	CE-IVD	96	7970362	Favorite
NGSgo®-AmpX v2 HLA-C Components HLA-C Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370362	Favorite
NGSgo®-AmpX v2 HLA-DRB1 Components HLA-DRB1 Amp Nuclease Free H2O GenDx-LongMix	IVD Canada	96	7770662	Favorite
NGSgo®-AmpX v2 HLA-DRB1 Components HLA-DRB1 Amp Nuclease Free H2O GenDx-LongMix	CE-IVD	96	7970662	Favorite
NGSgo®-AmpX v2 HLA-DRB1 Components HLA-DRB1 Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370662	Favorite
NGSgo®-AmpX v2 HLA-DRB1 Whole Gene Components HLA-DRB1 Whole Gene (except part of intron 1) Nuclease-Free H2O GenDx-LongMix	RUO	96	7370622	Favorite
NGSgo®-AmpX v2 HLA-DRB3/4/5 Components HLA-DRB3 Amp HLA-DRB4 Amp HLA DRB5 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7770762	Favorite
NGSgo®-AmpX v2 HLA-DRB3/4/5 Components HLA-DRB3 Amp HLA-DRB4 Amp HLA DRB5 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7970762	Favorite
NGSgo®-AmpX v2 HLA-DRB3/4/5 Components HLA-DRB3 Amp HLA-DRB4 Amp HLA DRB5 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7370762	Favorite
NGSgo®-AmpX v2 HLA-DQA1 Components HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7770862	Favorite
NGSgo®-AmpX v2 HLA-DQA1 Components HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7970862	Favorite
NGSgo®-AmpX v2 HLA-DQA1 Components HLA-DQA1 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7370862	Favorite
NGSgo®-AmpX v2 HLA-DQB1 Whole Gene Components HLA-DQB1 whole gene Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370512	Favorite
NGSgo®-AmpX v2 HLA-DQB1 Components HLA-DQB1 Amp Nuclease Free H2O GenDx-LongMix	IVD Canada	96	7770562	Favorite
NGSgo®-AmpX v2 HLA-DQB1 Components HLA-DQB1 Amp Nuclease Free H2O GenDx-LongMix	CE-IVD	96	7970562	Favorite
NGSgo®-AmpX v2 HLA-DQB1 Components HLA-DQB1 Amp Nuclease Free H2O GenDx-LongMix	RUO	96	7370562	Favorite
NGSgo®-AmpX v2 HLA-DPA1 Components HLA-DPA1 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7970962	Favorite
NGSgo®-AmpX v2 HLA-DPA1 Components HLA-DPA1 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7770962	Favorite
NGSgo®-AmpX v2 HLA-DPA1 Components HLA-DPA1 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7370962	Favorite
NGSgo®-AmpX v2 HLA-DPB1 Components HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	IVD Canada	96	7770462	Favorite
NGSgo®-AmpX v2 HLA-DPB1 Components HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	CE-IVD	96	7970462	Favorite
NGSgo®-AmpX v2 HLA-DPB1 Components HLA-DPB1 Amp Nuclease free H2O GenDx-LongMix	RUO	96	7370462	Favorite
NGSgo®-AmpX v2 DPB1 Whole Gene One (prototype) Components HLA-DPB1 F1 Amp	N/A	96	7270412	Favorite
NGSgo®-AmpX v2 HLA-G Components HLA-G Amp Nuclease free H2O GenDx-LongMix	RUO	96	7371062	Favorite
NGSgo®-AmpX v2 HLA-E (prototype) Components HLA-E Amp Nuclease Free H2O GenDx-LongMix	N/A	96	7271262	Favorite
NGSgo®-AmpX v2 HLA-F (prototype) Components HLA-F F1 Amp Nuclease Free H2O GenDx-LongMix	N/A	96	7271962	Favorite

Specifications

NGSgo-AmpX v2 and v3 feature exactly the same HLA gene coverage as NGSgo-AmpX. The whole gene is amplified for the Class I HLA genes HLA-A, B and C. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified. For -DRB1 and -DQB1 there are also options with more extended coverage amplifying the whole (or almost the whole) gene.

Fig 1. NGSgo-AmpX v2/AmpX v3 HLAGeneSuite (kit containing 11 loci) amplification coverage

Fig 2. NGSgo-AmpX v2 whole gene amplification coverage

To be supplied by the user:

Cold block
Pipettes and pipette tips with hydrophobic filters
Thermal cycler
Microcentrifuge
Vortex
PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
Agarose gel electrophoresis system

Downloads

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Others

Benefits

Description

Catalog

Filter

Status

Locus

61 Items

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DRB3/4/5, DQB1, DQA1, DPB1, DPA1

Components

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1

Components

NGSgo-AmpX v3 HLA-A

Components

NGSgo-AmpX v3 HLA-B

Components

NGSgo-AmpX v3 HLA-C

Components

NGSgo-AmpX v3 HLA-DRB1

Components

NGSgo-AmpX v3 HLA-DQB1

Components

NGSgo-AmpX v3 HLA-DPB1

Components

NGSgo-AmpX v3 HLA-DRB3/4/5

Components

NGSgo-AmpX v3 HLA-DPA1

Components

NGSgo-AmpX v3 HLA-DQA1

Components

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1, DPA1, DQA1, DRB3/4/5

Components

NGSgo-AmpX v3 HLA-A, B, C, DRB1, DQB1, DPB1

Components

NGSgo-AmpX v3 HLA-A

Components

NGSgo-AmpX v3 HLA-B

Components

NGSgo-AmpX v3 HLA-C

Components

NGSgo-AmpX v3 HLA-DRB1

Components

NGSgo-AmpX v3 HLA-DQB1

Components

NGSgo-AmpX v3 HLA-DPB1

Components

NGSgo-AmpX v3 DRB3/4/5

Components

NGSgo-AmpX v3 HLA-DPA1

Components

NGSgo-AmpX v3 HLA-DQA1

Components

NGSgo®-AmpX v2 HLAGeneSuite (11 loci)

Components

NGSgo®-AmpX v2 HLAGeneSuite (11 loci)

Components

NGSgo®-AmpX v2 HLAGeneSuite (11 loci)

Components

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

NGSgo®-AmpX v2 HLA-A, B, C, DRB1, DQB1, DPB1

Components

NGSgo®-AmpX v2 HLA-A

Components

NGSgo®-AmpX v2 HLA-A

Components

NGSgo®-AmpX v2 HLA-A

Components

NGSgo®-AmpX v2 HLA-B

Components

NGSgo®-AmpX v2 HLA-B

Components

NGSgo®-AmpX v2 HLA-B

Components

NGSgo®-AmpX v2 HLA-C

Components

NGSgo®-AmpX v2 HLA-C

Components

NGSgo®-AmpX v2 HLA-C

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)

NGSgo®-AmpX v2 HLAGeneSuite
(11 loci)